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flt3 l  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec flt3 l
    Flt3 L, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flt3+l/Human+Flt3-Ligand%2C+premium+grade/10__1016_slash_j__isci__2026__116736-656-33-34
    Average 95 stars, based on 58 article reviews
    flt3 l - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Stage IIIa Lung Cancer Treatment by the Combined Tomotherapy and Infusion of Autologous Peripheral-Blood-Mononuclear-Derived Lymphocytes: A Case Report of Aged Patient
    Article Snippet: .. The medium was supplemented with human recombinant IL-7, IL-15, SCF, IL-2, and Flt3-L (all substances from Miltenyi Biotec, Bergisch Gladbach, Germany) each in a final concentration of 20 ng/mL. ..

    Article Title: The activating receptor NKp65 is selectively expressed by human ILC3 and demarcates ILC3 from mature NK cells.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved.. 2 Innate lymphocytes comprise cytotoxic Natural Killer (NK) cells and tissue-resident innate lymphoid cells (ILC) that are subgrouped according to their cytokine profiles into group 1 ILC (ILC1), ILC2, and ILC3.

    Concentration Assay:

    Article Title: Stage IIIa Lung Cancer Treatment by the Combined Tomotherapy and Infusion of Autologous Peripheral-Blood-Mononuclear-Derived Lymphocytes: A Case Report of Aged Patient
    Article Snippet: .. The medium was supplemented with human recombinant IL-7, IL-15, SCF, IL-2, and Flt3-L (all substances from Miltenyi Biotec, Bergisch Gladbach, Germany) each in a final concentration of 20 ng/mL. ..

    Cell Culture:

    Article Title: Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations
    Article Snippet: For scRNA-seq experiments, human G-CSF-mobilized CD34 + HSPCs from adult healthy donors were purchased from the Fred Hutchinson Cancer Center and cultured in StemSpan SFEM II media (STEMCELL Technologies, 09655) supplemented with StemSpan CC110 (1×) (STEMCELL Technologies, 02697) and 100 μg ml −1 penicillin–streptomycin. .. For editing experiments, mobilized human peripheral blood CD34 + HSPCs (STEMCELL Technologies) were cultured in StemSpan SFEM II medium supplemented with 100 ng μl −1 each of FLT3-L, TPO and SCF (Miltenyi Biotec, 130-096-474, 130-095-745 and 130-096-491) as well as 750 nM SR1 and 35 nM UM729 (STEMCELL Technologies, 72342 and 72332). ..

    Article Title: Posttransplantation clonal dynamics of hematopoietic stem cells carrying prenatal and early‐life DNMT3A mutations
    Article Snippet: Live (7AAD‐negative) Lineage‐/CD11c‐/CD34+ HSPCs were index‐sorted as single cells into flat‐bottom 384‐well plates (Cat. No. 781182; Greiner). .. Cells were cultured in StemSpan SFEM II medium (Cat. No. 09655; Stem Cell Technologies) supplemented with SCF (100 ng/mL, Cat. No. 130‐096‐692; Miltenyi); FLT3‐L (100 ng/mL, Cat. No. 130‐096‐474; Miltenyi); TPO (50 ng/mL, Cat. No. 130‐095‐745; Miltenyi); IL‐6 (20 ng/mL, Cat. No. 130‐093‐929; Miltenyi) and IL‐3 (10 ng/mL, Cat. No. 130‐093‐909; Miltenyi); UM729 (500 nM, Cat. No. 72332; Stem Cell Technologies) and StemRegenin‐1 (750 nM, Cat. No. 72342; Stem Cell Technologies); and primocin (100 μg/mL, Cat. No. ant‐pm‐1; InvivoGen). ..

    Article Title: Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterations.
    Article Snippet: For scRNA-seq experiments, human G-CSF-mobilized CD34+ HSPCs from adult healthy donors were purchased from the Fred Hutchinson Cancer Center and cultured in StemSpan SFEM II media (STEMCELL Technologies, 09655) supplemented with StemSpan CC110 (1×) (STEMCELL Technologies, 02697) and 100 μg ml−1 penicillin–streptomycin. .. For editing experiments, mobilized human peripheral blood CD34+ HSPCs (STEMCELL Technologies) were cultured in StemSpan SFEM II medium supplemented with 100 ng μl−1 each of FLT3-L, TPO and SCF (Miltenyi Biotec, 130-096-474, 130-095-745 and 130-096-491) as well as 750 nM SR1 and 35 nM UM729 (STEMCELL Technologies, 72342 and 72332). ..

    Isolation:

    Article Title: ThymoSpheres culture: A model to study human polyclonal unconventional T cells
    Article Snippet: MS5‐DLL4 cells were cultured in Dulbecco's modified Eagle medium (Thermo Fisher Scientific, 41965039) supplemented with 10% fetal calf serum (Biowest, S1810), before being harvested using trypsin. .. 7 500 CD34 + HPCs isolated from human CB and 1 920 000 MS5‐DLL4 cells were resuspended in 500 μL Roswell Park Memorial Institute medium (Thermo Fisher Scientific, 52400025) complemented with 4% B‐27 supplement (Thermo Fisher Scientific, 17504044), 30 μM L‐ascorbic acid (Sigma‐Aldrich, 1713265258), 1% penicillin/streptomycin (Thermo Fisher Scientific, 15140122), 1% GlutaMAX supplement (Thermo Fisher Scientific, 35050061), FLT3‐L (5 ng/mL, Miltenyi, 130096480), and interleukin‐7 (5 ng/mL, Miltenyi, 130095362; ATO medium) and pipetted into the microwell‐containing wells. ..

    Article Title: ThymoSpheres culture: A model to study human polyclonal unconventional T cells.
    Article Snippet: MS5-DLL4 cells were cultured in Dulbecco’s modified Eagle medium (Thermo Fisher Scien- tific, 41965039) supplemented with 10% fetal calf serum (Biowest, S1810), before being harvested using trypsin. .. 7 500 CD34+ HPCs isolated from human CB and 1 920 000 MS5-DLL4 cells were resuspended in 500 μL Roswell Park Memorial Institute medium (Thermo Fisher Scientific, 52400025) complemented with 4% B-27 supplement (Thermo Fisher Scientific, 17504044), 30 μM L-ascorbic acid (Sigma-Aldrich, 1713265258), 1% penicillin/streptomycin (Thermo Fisher Scientific, 15140122), 1% GlutaMAX supplement (Thermo Fisher Scientific, 35050061), FLT3-L (5 ng/mL, Miltenyi, 130096480), and interleukin-7 (5 ng/mL, Miltenyi, 130095362; ATO medium) and pipetted into the microwell-containing wells. ..

    Stem Cell:

    Article Title: Posttransplantation clonal dynamics of hematopoietic stem cells carrying prenatal and early‐life DNMT3A mutations
    Article Snippet: Live (7AAD‐negative) Lineage‐/CD11c‐/CD34+ HSPCs were index‐sorted as single cells into flat‐bottom 384‐well plates (Cat. No. 781182; Greiner). .. Cells were cultured in StemSpan SFEM II medium (Cat. No. 09655; Stem Cell Technologies) supplemented with SCF (100 ng/mL, Cat. No. 130‐096‐692; Miltenyi); FLT3‐L (100 ng/mL, Cat. No. 130‐096‐474; Miltenyi); TPO (50 ng/mL, Cat. No. 130‐095‐745; Miltenyi); IL‐6 (20 ng/mL, Cat. No. 130‐093‐929; Miltenyi) and IL‐3 (10 ng/mL, Cat. No. 130‐093‐909; Miltenyi); UM729 (500 nM, Cat. No. 72332; Stem Cell Technologies) and StemRegenin‐1 (750 nM, Cat. No. 72342; Stem Cell Technologies); and primocin (100 μg/mL, Cat. No. ant‐pm‐1; InvivoGen). ..

    other:

    Article Title: Respiratory syncytial virus inhibits type I interferon signaling to maintain HLA-DM expression in CD1c+ dendritic cells
    Article Snippet: PBMCs were isolated using a blood Ficoll paque (GE Healthcare, cat#17-1440-02) gradient.



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